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    By Alpha|By Interest|Emeritus


Michael Uhler, Ph.D.
Research Professor, MBNI
Professor, Department of Biological Chemistry

5043 BSRB
109 Zina Pitcher Place
Ann Arbor, MI 48109-2200

 

muhler@umich.edu

(734) 647-3188

 

 

Current Research Interests:

The Uhler laboratory studies the role of protein phosphorylation in the nervous system.  Historically, the research has focused on the cAMP-dependent (PKA) and cGMP-dependent (PKG) protein kinases.  These kinases phosphorylate a large number of neuronal substrates including receptors , ion channels and transcription factors to regulate the function of these proteins.  During the characterization of transcriptional regulation by PKA and PKG, our laboratory developed the Surface Transfection and Expression Protocol (STEP) method for solid phase transfection and we have used it to identify novel regulatory sequences.  More recently, we have collaborated with the Turner laboratory to develop lines of P19 embryonic carcinoma cells which undergo neuronal differentiation after induction of neurogenic bHLH proteins such as Ascl1.  We are currently using these cell lines to characterize the role of PKA phosphorylation during neuronal differentiation.


Selected Publications:

Stansberry J, Baude EJ, Taylor MK, Chen PJ, Jin SW, Ellis RE, Uhler MD. A cGMP-dependent protein kinase is implicated in wild-type motility in C. elegans. J Neurochem. 76(4):1177-87. (2000)

Gamm DM, Barthel LK, Raymond PA, Uhler MD. Localization of cGMP-dependent protein kinase isoforms in mouse eye.  Invest Ophthalmol Vis Sci. 41(9):2766-73. (2000)

Taylor MK, Uhler MD.  The amino-terminal cyclic nucleotide binding site of the type II cGMP-dependent protein kinase is essential for full cyclic nucleotide-dependent activation. J Biol Chem. 275(36):28053-62. (2000)

Taylor MK, Ahmed R, Begley M, Uhler MD. Autoinhibition and isoform-specific dominant negative inhibition of the type II cGMP-dependent protein kinase.  J Biol Chem. 277(40):37242-53. (2002)

Lum H, Hao Z, Gayle D, Kumar P, Patterson CE, Uhler MD. Vascular endothelial cells express isoforms of protein kinase A inhibitor.  Am J Physiol Cell Physiol. 282(1):C59-66. (2002)

Redmond TM, Ren X, Kubish G, Atkins S, Low S, Uhler MD. Microarray transfection analysis of transcriptional regulation by cAMP-dependent protein kinase. Mol Cell Proteomics. 3(8):770-9. (2004)

Zhang L, Duan CJ, Binkley C, Li G, Uhler MD, Logsdon CD, Simeone DM. A transforming growth factor beta-induced Smad3/Smad4 complex directly activates protein kinase A. Mol Cell Biol. 24(5):2169-80. (2004)

Ren X, Uhler MD.  Microarray transfection analysis of conserved genomic sequences from three immediate early genes. Genomics. 93(2):159-68. (2009)

Huang HS, Kubish GM, Redmond TM, Turner DL, Thompson RC, Murphy GG, Uhler, MD. Direct transcriptional induction of Gadd45gamma by Ascl1 during neuronal differentiation. Mol Cell Neurosci. 44(3):282-96. (2010)

 

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