Current Research Interests:
The Uhler laboratory studies the role of neuronal signaling in the context of human neurologic and psychiatric disorders. We have developed a number of tools to study gene expression in neurons derived from mouse and human stem cells. We derive various neuronal populations using expression of neurogenic transcription factors to produce excitatory and inhibitory neurons. Using these neurons, we have defined signal transduction cascades for fibroblast growth factors (FGFs) and identified genes regulated by FGFs that alter neuronal function. We are currently deriving neuronal populations from patients with major depressive disorder (MDD) to determine if FGF signaling is altered in MDD. We are also generating neuronal populations to understand the role of the Tau protein in Alzheimer's disease.
Ramakrishnan, S.K., Zhang, H., Takahashi, S., Centofanti, B., Periyasamay, S., Weisz, K. Chen, Z., Uhler, M.D. , Rui, L., Gonzalez, F.J. and Shah, Y.M. (2016) HIF2alpha is an essential molecular brake for postprandial hepatic glucagon response independent of insulin signaling. Cell Metab. 23(3) 505-16.
Huang, H.S., Redmond, T.M., Kubish, G.M., Gupta, S., Thompson, R.C., Turner, D.L. and Uhler, M.D. (2015) Transcriptional regulatory events initiated by Ascl1 and Neurog2 during neuronal differentiation of P19 embryonic carcinoma cells. J Mol. Neurosci. 55(3):684-705.
Yang, H., Li, G., Wu, J.J., Uhler, M.D. and Simeone, D.M. (2013) Protein kinase A modulates transforming growth factor-b signaling through a direct interaction with Smad4 protein. J. Biol. Chem. 288(12): 8737-49.
Zhang, H., Deo, M., Thompson, R.C., Uhler, M.D. and Turner, D.L. (2012) Negative regulation of Yap during neuronal differentiation. Dev. Biol. 361(1): 103-15.
Huang, H.S., Turner, D.L., Thompson, R.C. and Uhler, M.D. (2012) Ascl1-induced neuronal differentiation of P19 cells requires expression of a specific inhibitor protein of cAMP-dependent protein kinase. J. Neuochem. 120(5): 667-83.
Redmond, T.M. and Uhler, M.D. Cell-based Microarrays for the Study of Transcriptional Regulation in Multiple Cell Types. (2011). Methods in Molecular Biology 706: 41-52.
Huang, H.S., Kubish, G.M., Redmond, T.M., Turner, D.L., Thompson, R.C., Murphy, G.G. and Uhler, M.D. (2010) Direct transcriptional induction of Gadd45gamma by Ascl1 during neuronal differentiation. Mol. Cell Neurosci. 44(3): 282-296.
Ren, X. and Uhler, M.D. (2009) Microarray transfection analysis of conserved sequences from three immediate early genes. Genomics 93(2): 159-168.